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Category Archives: Technique
Molecular Techniques
Molecular Techniques (eg. PCR)
- Fresh tissue is snap-frozen using liquid nitrogen; some techniques permit the use of paraffin-embedded tissue which are then submitted to molecular techniques
Advantages
• Extremely sensitive
• Useful for detection of oncogenes, fusion genes, minimal residual disease (especially in follicular lymphoma and CML)
Disadvantages
• Sampling error may result in false positives and negatives
• Poor fixation may be an issue in paraffin-based techniques
• Expensive; may not be available in some centres
Posted in Technique
Tagged , Molecular diagnostic test, Molecular technique, Molecular tests, pcr, polymerase chain reaction
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Flow Cytometry
Flow Cytometry
- Fresh tissue is placed in RPMI or MEM medium to create a cell suspension
- Aliquots are treated with fluorochrome-conjugated antibodies and passed through a flow cytometry apparatus
Advantages
• Available to most community hospitals
• Useful for analyzing small lymphocytic infiltrates and leukemic processes
Disadvantages
• Sampling errors and cell mortality may minimize its sensitivity
Posted in Technique
Tagged , Flow cytometry, Flow cytometry advantages, Flow cytometry diagnosis, Flow cytometry disadvantages
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