Calculations

Derivation of Binding Constants - http://www.its.caltech.edu/~bjorker/Protocols/Equilibrium_Dialysis.pdf Bjorkman Lab. Derivation of Binding Constants by Inhibition Equilibrium Dialysis Preparing Reagents: Buffer: Use 1 x PBS with 0.5% gelatin and 0.02% azide. Filter through a 0.2 μm filter and store at 4oC. Peptides: Dissolve the inhibitor peptides in water or PBS. If the peptide contains tyrosine, run a scan on the spectrophotometer from 310 nm to 210 nm. Determine the molar concentration by dividing the A274 by - [Read more]