Western Blot

Western Blot Protocol - http://www.its.caltech.edu/~bjorker/Protocols/Western%20blot%20KHT.pdf Bjorkman Lab Protocols. Western blot 1. Run gel at 200 V, 50 min. 2. Prepare for transfer of gel: a. cut Hybond ECL nitrocellulose membrane to size of gel b. cut four pieces of Whatman 3 MM filter paper to size of gel c. make transfer buffer (10% methanol in Laemmli buffer, without SDS) d. pre-soak Hybond ECL nitrocellulose membrane in transfer buffer (15 min, RT) 3. Set up transfer: - [Read more]