Protein Electrophoresis

SDS-PAGE - http://www.its.caltech.edu/~bjorker/Protocols/Western_blot.pdf Bjorkman Lab Protocols. SDS-PAGE Stock solutions: SDS-PAGE itself Acrylamide: 30% acrylamide, 0.8% bis-acrylamide. Store at 4 C in the dark. APS: 25 mg of ammonium persulfate in 100 ml of H2O. Store at 4 C. 8.8 buffer: 1.5 M tris pH=8.8, 0.4% SDS 6.8 buffer: 0.5 M tris pH=6.8, 0.4% SDS - [Read more]

SDS-PAGE Silver Staining - http://www.its.caltech.edu/~bjorker/Protocols/Silver_staining_of_SDS-PAGE.pdf Bjorkman Lab Protocols. Silver staining of SDS-PAGEs NOTES: The reagents should be prepared when they are going to be used. It is a good idea to stain the gel previously with Coomasie The incubations are at room temperature with shaking 1- Incubate 30 min in 40% methanol with 10% acetic acid 2- Incubate 30 min in 40% methanol with 10% acetic acid - [Read more]

TCA Protein Precipitation - http://www.its.caltech.edu/~bjorker/Protocols/TCA_ppt_protocol.pdf Bjorkman Lab Protocols. TCA protein precipitation protocol (orginally from Luis Sanchez) revised: October 10, 2001 author: clw Stock Solutions: 100% (w/v) Trichloroacetic acid (TCA) recipe: dissolve 500g TCA (as shipped) into 350 ml dH2O, store at RT. (for details, check Maniatias under TCA ppt ) Precipitation Protocol: - [Read more]