Microsatellite

Microsatellite Library Protocol - http://people.bu.edu/pbarber/Web%20Protocols/Microsatellite%20Library.pdf Outline of Microsatellite Library Protocol DNA Extraction (1 overnight [optional], 3 hrs setup) Digestion and Size Fractionation (8 hours) Linker Creation (15 minutes) Linker Ligation (15 minute setup, 1 overnight) - [Read more]

Microsatellite Protocol - http://people.bu.edu/pbarber/Web%20Protocols/Microsatellite%20gel%20protocol.pdf Microsatellite Protocol Prepare gel: 1. Pour Acrylamide gel (mix up 25 mL Acrylamide solution, 125 uL APS, 17 uL TEMED) 2. Wait 30 minutes, add damp paper towel to open bottom of plates to keep from drying out.3. Wait 1:30 hours longer (2 hours total) for gel to finish setting up. - [Read more]