Flow Cytometry

FACS Analysis - http://www.lbl.gov/LBL-Programs/lifesciences/BissellLab/labprotocols/facs.htm Bissell Lab FACS Analysis 1.     Harvest cell using 0.25% trypsin 2.     Resuspend in 1ml DMEM/F-12 media with Trypsin inhibitor 3.     Placed in a 1.5 ml micro centrifuge tube and spin down 4.     Wash with FACS buffer (1XPBS, 5% FBS, and 0.1% sodium azide) in ice - [Read more]