Epithelial Cell Isolation

Epithelial Cell Isolation - http://www.lbl.gov/LBL-Programs/lifesciences/BissellLab/labprotocols/primarymammaryepithelialcellisolation.htm Bisell Lab. Primary Mammary Epithelial Isolation Mince glands with two razor blades on sterile surface. Make collagenase solution and prewarm at 37C (shake occasionally)- filter sterilize after the collagenase has gone into solution through a .2-micron filter. Put solution into fifty ml falcon. Must make this media fresh (collagenase will inactivate at high temperatures and over long periods of time): - [Read more]

Primary Epithelial Cells Isolation - http://www.lbl.gov/LBL-Programs/lifesciences/BissellLab/labprotocols/primarycellprep.htm Isolation of Primary Epithelial Cells Digestion: In Collagenase Medium    1. Sacrifice mouse with CO2 inhalation and cervical dislocation.    2. Put whole mouse in 70%ETOH.    3. Dissect out 3rd, 4th and 5th pair of MG, (avoid muscle and tendons) with sterile instruments. Remove lymph nodes in 4th glands. - [Read more]