ELISA

Anti FcRn ELISA Protocol - http://www.its.caltech.edu/~bjorker/Protocols/Anti_FcRn_ELISA.pdf Bjorkman Lab Protocols. Anti FcRn ELISA Protocol 1) Coat plate with IG3 diluted 1/1000 (use the 12/1/95 Ab) into PBS. 100 ml/well, Incubate overnight at 4 C. 2) Wash three times with PBS/0.05% Tween pH 7-7.6 (Take excess out by taping the plate against a paper towel). 3) Incubate supernatants, 200 ml/well for 1 hour @ 37 C. - [Read more]

Monoclonal Antibody Screening by Direct ELISA - http://www.its.caltech.edu/~bjorker/Protocols/Siliconized%20coverslips.pdf Bjorkman Labs Protocols. Monoclonal Antibody Screening by Direct ELISA Stock solutions: PBS: 20 mM NaPi pH=7.5, 150 mM NaCl PT: 0.1% Tween 20 in PBS Blocking solution: 3% BSA in PT. Store at -20 C Developing solution: 10 mg o-phenylenediamine in 25 ml of a buffer with pH=5.5 (say, 20 ml of H2O + 5.16 ml of 0.5 M Na2HPO4 + 645 μl of 1.5 M citric acid) + 12 μl of 30% H2O2. - [Read more]