DNA Extraction

Chelex Extraction Protocol - http://people.bu.edu/pbarber/Web%20Protocols/Protocol2.pdf Extraction Protocol: Chelex This is a fast, cheap, and effective method of DNA extraction. Because this is the first step towards PCR and amplifying your template DNA, you must maintain excellent sterile technique to prevent the contamination of your DNA extractions. Always use a negative chelex control (below) to evaluate your technique during the extraction phase. - [Read more]

Chelex Protocol - http://people.bu.edu/pbarber/Web%20Protocols/Chelex%20Protocol-SLB.pdf Preparation of Chelex Mixture: 1. With Bleach, sterilize a dry reagent spatula, and a small magnetic stir bar. 2. Prepare a 5-10% by weight slurry of Chelex 100 Resin (Biorad part 143- 3832,100-200 mesh Chelex, sodium form) and UV sterilized HPLC water. The most effective way to do this is to take a 50 ml sterile falcon tube, place in on a scale inside a small beaker and zero the scale. - [Read more]