Chromatin Immunoprecipitation

Chromatin immunoprecipitation assay protocol - http://www.lbl.gov/LBL-Programs/lifesciences/BissellLab/labprotocols/chip.htm ChIP assay: Chromatin immunoprecipitation assay was performed according to the protocol of ChIP assay kit (Upstate Biotechnology, Lake Placid, NY). 1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. - [Read more]

Immunoprecipitation Screening Protocol - http://www.its.caltech.edu/~bjorker/Protocols/Immunopptns%20-%20HFE.pdf Bjorkman Lab Protocols. COLD IMMUNOPRECIPITATIONS FOR SCREENING OF SOLUBLE HFE MUTANT TRANSFECTED CELL SUPERNATANTS Christina Milburn 10/27/97 ANTIBODIES AVAILABLE This protocol calls for the 1C3.1G4 mAb (anti-HFE heavy chain), because that's what I've tried, - [Read more]