Biacore

Binding Experiments - http://www.its.caltech.edu/~bjorker/Protocols/Biacore.pdf Suggestions for Successful Binding Experiments Bjorkman Lab These suggestions are based on personal experiences with the instrument and methods obtained or derived from Dave Myszka’s group. They pertain only to the operation and maintenance of the machine. Experimental design is up to you. Example methods files are in the Bjorkman directory on the PC. These are actual methods files I’ve used for various data collections. - [Read more]

Covalent oriented coupling - http://www.its.caltech.edu/~bjorker/Protocols/Cov_orien_coup.pdf Covalent oriented coupling Bjorkman Lab 1) Couple your capture antibody 2) flow over your his-tagged protein 3) Mix the NHS/EDC reagents yourself (I use the same NHS and EDC aliquots that I also use for activating chip surfaces so you don't have to make those separately, just pull some aliquots from the freezer). 4) Inject the NHS/EDC over the flow-cell(s) of interest. - [Read more]