Baculovirus

Culture Media Recipe - http://www.its.caltech.edu/~bjorker/Protocols/Sf9_Spinner_Media_Recipe.pdf Sf9 Spinner Culture Media Recipe revised October 10, 2001 Bjorkman Lab. -CLW Media to be use with Sf9/Sf21/HiFive insect spinner flask culture. Can also be used for plate cultures, though Pluronic Acid, Gentamicin, and Fungizone may inhibit cell growth/viability and decrease protein expression/secretion. Try using protein free (serum free) media when optimizing protein expression levels. - [Read more]

Recombinant Baculovirus Generation by Co-transfection - http://www.its.caltech.edu/~bjorker/Protocols/Baculovirus-transfection.pdf Generation of recombinant baculoviruses by co-transfection Bjorkman Lab. (modified from PharMingen, WS, 6-10-96) For co-transfection prepare at least 10mg of highly purified plasmid DNA. Spodoptera frugiperda cells are sensitive to some contaminants found in crude plasmid preparations, which cannot be removed by phenol/chloroform extraction or ethanol precipitation. The only consistently reliable method for plasmid purification is cesium - [Read more]