In Situ Hybridization

In Situ Hybridization Protocol - http://www.lbl.gov/LBL-Programs/lifesciences/BissellLab/labprotocols/insituhybridization.htm Bisell Lab. In Situ Hybridization Method on Mammary Sections Using DIG Probes Day 1 1.Dewax and Rehydrate paraffin embedded sections a.2 X 2 minutes toluene[2] b.2 min 100% ETOH c.2 min in 95% ETOH d.2 min in 80% ETOH e.2 min in 70% ETOH f.2 X 2 min in 2X SSC buffer 2. Proteinase K Digestion - [Read more]