Tissue Extraction Protocols

Analysis of murine Bone Marrow: specimen choice, sampling and processing. - http://icg.cpmc.columbia.edu/cattoretti/Protocol/MousePathology/AnalysisMurineBM.html Histologic analysis of murine bone marrow is a necessary complement to flow cytometric or in vitro analysis. - [Read more]

Blood collection using the saphenous vein: An alternative to retro-orbital collection - http://www.uib.no/vivariet/mou_blood/Blood_coll_mice_.html Blood collection using the saphenous vein: An alternative to retro-orbital collection - [Read more]

CAM preparation Protocol - http://www.pharma.ethz.ch/institute_groups/biomacromolecules/protocols/cam CAM preparation Protocol - [Read more]

Collection of Peritoneal Cells - http://icg.cpmc.columbia.edu/cattoretti/Protocol/MousePathology/CollectPerC.html Collection of Peritoneal Cells Protocol. This protocol has been orginated by Alan M Stall in the Leon and Lenore Herzenberg's Lab at Stanford . Shaking the mouse was found to be necessary for optimum yield of B1 cells. - [Read more]

Decalcification of Animal Tissue Protocol - http://molecool.wustl.edu/ornitzlab/PDFs/Protocols/Decalcification.pdf Decalcification of Animal Tissue Protocol - [Read more]

Dissociation of spleen and hemopoietic tissue. - http://icg.cpmc.columbia.edu/cattoretti/Protocol/MousePathology/DissociationSpleen.html Dissociation of spleen and hemopoietic tissue protocol. You need to buy glass slides with frosted, sandblasted ends (Fisher Scientific, Catalog N. 12-552). Frosting by painting (e.g.Superfrost) should not be used. Pre-clean the slides by rubbing the edges of the slides against each other in medium, to get rid of the glass powder. Slides can be autoclaved in advance for sterile dissociation. - [Read more]

Isolation of T4 cells from xenograft tumors - http://www.lbl.gov/lifesciences/BissellLab/labprotocols/isolationofcellsfromtumorxenografts.htm Isolation of T4 cells from xenograft tumors - [Read more]

Pathology and Autopsy of a Mouse - http://icg.cpmc.columbia.edu/cattoretti/Protocol/MousePathology/mouseTissue.html Pathology and Autopsy of a Mouse. Tissue embedding in paraffin, Storage of smears, and slides Formalin fixation. - [Read more]

Penn State Veterinary Medicine Animal Surgery - http://cal.vet.upenn.edu/ Penn State Veterinary Medicine Animal Surgery Protocols and Methods, and General Information - [Read more]

Protocols for the preparation of tumour cells for subcutaneous injections in mice - http://www.pharma.ethz.ch/institute_groups/biomacromolecules/protocols/tumorcell Protocols for the preparation of tumour cells for subcutaneous injections in mice. - [Read more]