Methylation polymerase chain reaction (PCR) information, links, protocols, methods, techniques and articles. Methylation PCR is used to detect the presence of methyl-cytosines within DNA sequences accurately and quickly. It is often used as a screening tool when primers have been created to study a specific area of DNA. For more wide-spread analysis of DNA methylation, sodium bisufite DNA treatment and subsequent sequencing is mroe appropriate.
Methylation PCR Primer Design (1) |
| methBLAST - http://medgen.ugent.be/methBLAST/ methBLAST is a program that allows you to check your primers for bisulfite converted DNA by blasting them against the unmethylated and methylated genomic sequences of man, mouse and rat. - [Read more] |
| MethPrimer - http://www.urogene.org/methprimer/ MethPrimer is a program that is able to design bisulfite-conversion-based Methylation PCR Primers. MethPrimer can design primers for two types of bisulfite PCR: 1) Methylation-Specific PCR (MSP) and 2) Bisulfite-Sequencing PCR (BSP) or Bisulfite-Restriction PCR. - [Read more] |
| Methylation PCR - http://www.mdanderson.org/departments/methylation/display.cfm?id=0CB11381-59AE-4D90-AC0B343F2D646EDD&method=displayFull Methylation-Specific PCR. Protocol by James Herman. Basic Protocol, Critical parameters. The University of Texas M. D. Anderson Cancer Center. DNA Methylation in Cancer |
| PCR amplification of DNA methylated with the DamID technique - http://www.epigenome-noe.net/researchtools/protocol.php?protid=16 PCR amplification of DNA methylated with the DamID technique developed by Frauke Greil from The Netherlands Cancer Institute - [Read more] |
| Promoter Methylation PCR - http://www.panomics.com/downloads/MP1100UM100505.pdf Find information on promoter methylation PCR through this manual and booklet on promoter methylation PCR kit. - [Read more] |
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