Biology Disease

Dissociation of spleen and hemopoietic tissue. - Link

Information 

Title:

Dissociation of spleen and hemopoietic tissue.

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http://icg.cpmc.columbia.edu/cattoretti/Protocol/MousePathology/DissociationSpleen.html

Description:

Dissociation of spleen and hemopoietic tissue protocol. You need to buy glass slides with frosted, sandblasted ends (Fisher Scientific, Catalog N. 12-552). Frosting by painting (e.g.Superfrost) should not be used. Pre-clean the slides by rubbing the edges of the slides against each other in medium, to get rid of the glass powder. Slides can be autoclaved in advance for sterile dissociation.

Category:

Molecular Biology: Tissue Extraction Protocols

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