Biology Disease

LIPOFECTION Protocol - Link

Information 

Title:

LIPOFECTION Protocol

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http://www.its.caltech.edu/~bjorker/Protocols/Lipofection_Procedure.pdf

Description:

Bjorkman Lab Protocols.
LIPOFECTION PROCEDURE- CHO (PF)
Complete medium: 5-10% FCS, P/S, glutamine
Selection medium: 10% dFCS (dialyzed), P/S, no glutamine, 0-100 mM MSX
Cells: Use CHO cells that have been in culture less than one month and have been
maintained subconfluent for several passages in advance. (A 10 cm plate of CHO cells
which have just reached confluence has about 107 cells on it. Densities of 2-5 X 107

Category:

Molecular Biology: Transfection

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