Biology Disease

HLA-B27 Binding Assay - Link

Information 

Title:

HLA-B27 Binding Assay

 -

http://www.its.caltech.edu/~bjorker/Protocols/B27_binding_assay.pdf

Description:

Bjorkman Lab.
HLA-B27 Binding Assay
1) As of today the B27 protein concentration = 9 mg/mL
2) Dilute the protein 2-fold with 1xPBS:
75 μl B27 (9 mg/mL)
75 μl 1xPBS
2 μl 0.1M DTT
3) Incubate on ice for 5 minutes.
4) Meanwhile, prepare the spin columns (Biospin 6 and Biospin 30 from Biorad;
they are stored in the cold room).
Biospin -6 column equilibration:

Category:

Immunology

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