Biology Disease

Derivation of Binding Constants - Link

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Title:

Derivation of Binding Constants

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http://www.its.caltech.edu/~bjorker/Protocols/Equilibrium_Dialysis.pdf

Description:

Bjorkman Lab. Derivation of Binding Constants by Inhibition Equilibrium Dialysis
Preparing Reagents:
Buffer: Use 1 x PBS with 0.5% gelatin and 0.02% azide. Filter through a 0.2 μm
filter and store at 4oC.
Peptides: Dissolve the inhibitor peptides in water or PBS. If the peptide contains
tyrosine, run a scan on the spectrophotometer from 310 nm to 210
nm. Determine the molar concentration by dividing the A274 by

Category:

Research Tools: Calculations

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Biology Disease

Biology Disease

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