Biology Disease

Agar Colony Formation Assay Protocol - Link

Information 

Title:

Agar Colony Formation Assay Protocol

 -

http://www.lbl.gov/LBL-Programs/lifesciences/BissellLab/labprotocols/softagar.htm

Description:

Bissell Lab.
SOFT AGAR ASSAY FOR COLONY FORMATION
Note: All volumes are calculated to cater for four plates per point.

Base Agar
1. Melt 1% Agar (DNA grade) in microwave, cool to 40�C in a water bath. Warm 2X DMEM/F12 + additives to 40�C in water bath. Allow at least 30 minutes for temperature to equilibrate.
2. Mix equal volumes of the two solutions to give 0.5% Agar + 1X DMEM/F12 + additives.

Category:

Cell Culture: Soft Agar Assay for Colony Formation

Number Hits:26
 
Biology Disease

Biology Disease

Find information, links, pictures and protocols on biology, molecular biology, diseases and more, within our molecular biology link directory and images within our picture gallery.