Biology Disease

Isolation of cells from xenograft tumors - Link

Information 

Title:

Isolation of cells from xenograft tumors

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http://www.lbl.gov/LBL-Programs/lifesciences/BissellLab/labprotocols/isolationofcellsfromtumorxenografts.htm

Description:

Bissell Lab.
Isolation of T4 cells from xenograft tumors protocol:
1. Put mouse down
2. Wash skin by dipping mice into ETOH
3. Remove tumor and put into 5ml PBS containing Fungizone and Penstrep
4. Swirl around to rinse tumor and pour on to a sterile plate
5. Remove tumor from PBS place on sterile plate or lid
6. Remove any skin-rinse a second time if needed

Category:

Cell Culture: Cell Preparation and Isolation

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