Biology Disease

On Top Cell Culture Protocol - Link

Information 

Title:

On Top Cell Culture Protocol

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http://www.lbl.gov/LBL-Programs/lifesciences/BissellLab/labprotocols/ontop.htm

Description:

Bissell Lab. On Top cell culture protocol.
Before splitting the cells, prepare the
Matrigel preparation
Thaw on the ice O/N (keep it in the fridge)
1 bottle thawed: 1 month at 0C or refrozen once (make aliquots)
Using cold tips and cold dishes can help to spread more homogeneously the matrigel

Category:

Cell Culture: Cell Culture Procedures

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Biology Disease

Biology Disease

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