Biology Disease

Freezing Cells Protocol - Link

Information 

Title:

Freezing Cells Protocol

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http://www.lbl.gov/LBL-Programs/lifesciences/BissellLab/labprotocols/freeze.htm

Description:

Bissell Lab. Cell Culture: Freezing cells Protocol
After steps 11 from the protocols splitting and passing cells
1) Centrifuge cells 5 minutes at 1000 rpm (=117xg)
2) Aspirate supernatant.
3) Disperse pellet.
4) Add freezing medium (10% glycerol, 15% fetal calf serum, 75% DMEM/F12) to a concentration of 1.5X106 S1 cells/ml or 0.75X106 T4 cells/ml (that is, 1X density for a T75 flask per ml freezing media).

Category:

Cell Culture: Cryopreservation of Cell Lines

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Biology Disease

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