Soft Agar Assay for Colony Formation

Agar Colony Formation Assay Protocol - http://www.lbl.gov/LBL-Programs/lifesciences/BissellLab/labprotocols/softagar.htm Bissell Lab. SOFT AGAR ASSAY FOR COLONY FORMATION Note: All volumes are calculated to cater for four plates per point. Base Agar 1. Melt 1% Agar (DNA grade) in microwave, cool to 40�C in a water bath. Warm 2X DMEM/F12 + additives to 40�C in water bath. Allow at least 30 minutes for temperature to equilibrate. 2. Mix equal volumes of the two solutions to give 0.5% Agar + 1X DMEM/F12 + additives. - [Read more]