Cryopreservation of Cell Lines

Cryopreservation of Cell Lines - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Cell_Culture/Key_Resources/ECACC_Handbook/Cell_Culture_Techniques_12.html Cryopreservation of Cell Lines - [Read more]

Freezing Cells Protocol - http://www.lbl.gov/LBL-Programs/lifesciences/BissellLab/labprotocols/freeze.htm Bissell Lab. Cell Culture: Freezing cells Protocol After steps 11 from the protocols splitting and passing cells 1) Centrifuge cells 5 minutes at 1000 rpm (=117xg) 2) Aspirate supernatant. 3) Disperse pellet. 4) Add freezing medium (10% glycerol, 15% fetal calf serum, 75% DMEM/F12) to a concentration of 1.5X106 S1 cells/ml or 0.75X106 T4 cells/ml (that is, 1X density for a T75 flask per ml freezing media). - [Read more]