Subculture of Suspension Cell Lines, links to protocols, methods, techniques and procedures. In general terms cultures derived from blood grow in suspension. Cells may grow as single cells or in clumps. For these types of lines subculture by dilution is relatively easy. But for lines that grow in clumps it may be necessary to bring the cells into a single cell suspension by centrifugation and resuspension by pipetting in a smaller volume before counting.
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