Protocols and information links on Cell culture quality control, antibiotic treatments to prevent and treat contamination, and cleaning cells from contaminants, such as yeast.
| Authentication of Cell Lines - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Cell_Culture/Key_Resources/ECACC_Handbook/Cell_Culture_Techniques_10.html#Authentication%20of%20Cell%20Lines Authentication of Cell Lines - from the Fundamental Techniques in Cell Culture Laboratory Handbook. - [Read more] |
| Decontamination of cells from the yeast - http://www.geocities.com/mesentsev/Biblioteque/decontamination.html Decontamination of cells from the yeast - a great protocol for use when you are in trouble from yeast. - [Read more] |
| How to decontaminate tissue culture samples - by Invitrogen - http://invitrogen.custhelp.com/cgi-bin/invitrogen.cfg/php/enduser/std_adp.php?p_sid=HczfJTng&p_lva=1&p_faqid=69 How to decontaminate tissue culture samples - by Invitrogen - a great protocol and procedure to follow for decontamination. - [Read more] |
| Method: Removal of Yeast Contamination from Lymphoblast Cultures - http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc8.html Method: Removal of Yeast Contamination from Lymphoblast Cultures - This method is advantageous for saving the occasional cultures that become contaminated. Yeast contaminated cultures will appear cloudy when slightly shaken and lymphocytes will not cluster together as much as normal. If cultures are suspect, a drop of culture can be streaked on a YPD media plate to check for growth of yeast colonies, or a 5 ml sample can be taken to Barnes Diagnostic Center for identification of yeast strain. - [Read more] |
| Mycoplasma Detection Using DNA Staining - http://www.corning.com/Lifesciences/technical_information/techDocs/Mycoplasma_testing_protocol.pdf Mycoplasma Detection Using DNA Staining - [Read more] |
| Understanding and Managing Cell Culture Contamination - http://www.corning.com/Lifesciences/technical_information/techDocs/cccontamination.pdf A 24 page PDF on Understanding and Managing Cell Culture Contamination. Very informative and descriptive detial and outline on culture contamination. - [Read more] |
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