Anchorage Independence Assay

Anchorage Independence Assay Protocol - http://www.lbl.gov/LBL-Programs/lifesciences/BissellLab/labprotocols/anchorage.htm Anchorage Independence Assay using Methylcellulose / Polyhema Materials: Methyl Cellulose (Fisher, Cat #M-352) Polyhema (Sigma, Cat #P3932) I. Polyhema coating procedure (10X solution =120 mg/ml) 1. Dissolve 2.4 g in 20 ml 95% ETOH by rotating ~ 8hr @ 65 C 2.Dilute 1:10 in 95% ETOH (final conc. = 12mg/ml). Coat plates with 4ml/100 mm dish 1.5 ml/ 60mm - [Read more]